The Sistine Chapel Choir, as depicted in the movie "The Sistine Chapel Choir," traditionally consists of around 20 to 30 singers. This prestigious choir is known for its rich history and significant role in the Vatican's liturgical ceremonies, performing sacred music that dates back centuries. The choir includes both adult male singers and boy sopranos, contributing to its unique and powerful sound.

In the movie "The Sistine Chapel Choir," the singers featured are members of the actual Sistine Chapel Choir, known for their rich history and connection to the Vatican. The choir comprises male voices, traditionally featuring boys and men who perform sacred music, particularly during liturgical services in St. Peter's Basilica. The film highlights their unique vocal techniques, training, and the significance of their music within the context of the Catholic Church and its traditions.

Taking pictures in the Sistine Chapel is prohibited primarily to protect the artwork and maintain the sanctity of the space. Flash photography can damage the delicate frescoes, and the noise from cameras can disrupt the solemn atmosphere. Additionally, the Vatican aims to control the experience for visitors, ensuring they engage with the art and architecture without distractions. The ban also helps preserve the chapel’s integrity and promotes respectful behavior within this significant cultural and religious site.

At Pope John Paul II's funeral in April 2005, the Sistine Chapel Choir performed a significant role. This renowned choir, which is an integral part of the Vatican's musical heritage, sang various liturgical pieces during the Mass and the funeral rites. Their performance included traditional hymns and chants, contributing to the solemn and reverent atmosphere of the ceremony. The choir's participation highlighted its historical importance in the Catholic Church and its tradition of sacred music.