In the movie "Don't Think Twice," the title references Bob Dylan's song "Don't Think Twice, It's All Right." This song has been covered by numerous artists over the years, including the likes of Joan Baez, Johnny Rivers, and Eric Burdon. Each artist brings their unique style to the song, contributing to its enduring popularity. The film explores themes of friendship and the struggles of pursuing dreams, paralleling the reflective nature of Dylan's lyrics.

Bob Dylan wrote "Don't Think Twice, It's Alright," which was released in 1963 on his album "The Freewheelin' Bob Dylan." The song is often interpreted as a bittersweet farewell, reflecting themes of love, loss, and moving on. While it shares a title with the film "Don't Think Twice," the movie, directed by Mike Birbiglia and released in 2016, explores the dynamics of a comedy troupe facing changes in their lives and careers. The song's themes resonate with the film's exploration of relationships and personal growth.

In the movie "Don't Think Twice," the phrase "don't think twice" reflects the idea of seizing opportunities and making bold choices without overthinking or second-guessing oneself. It encourages characters to pursue their dreams and passions, particularly in the context of improv comedy and the uncertainty of artistic careers. This theme emphasizes the importance of taking risks and embracing spontaneity, as hesitation can lead to missed chances in both personal and professional life.

"Don't Think Twice" is not directly based on a true story, but it draws inspiration from the experiences of its writer and director, Mike Birbiglia, and the struggles of improv comedians. The film explores themes of friendship, ambition, and the challenges of pursuing a career in comedy, reflecting the real-life dynamics and uncertainties faced by performers in the industry. While the characters and plot are fictional, they resonate with the experiences of many in the world of improv and comedy.